A Few Examples of Higgins Analytical Proto200 & Proto 300 Column Comparisons to Equivalent Vydac Columns

Click on the chromatogram for details and full size view

Hemoglobin

Mouse Hb α,β major/minor with
Human Sickle Hb

Data courtesy of Sandra M. Suzuka, Albert Einstein Hematology
and
The Nest Group, Southborough, MA
Phosphopeptides

Phosphopeptide Purity Analysis

Proto 200 C18 compared to Vydac 238EV and Vydac218TP

Courtesy of
The Nest Group, Southborough, MA
Peptide Analysis

Proto 200 C18 vs Vydac 218TP54
for Peptide Analysis

Data Courtesy of Jessica Shu, University of CA Berkeley
and
The Nest Group, Southborough, MA
Peptide Fragments

Comparison of Proto 200 C4 to
Vydac 218TP C18

Courtesy of Mahesh K Karwa, PharmaForce, Inc, Columbus OH
and
The Nest Group, Southborough, MA
Tryptic Digests

Proto 300 C4 5µm compared to:
Vydac 208TP52 5µm and Zorbax C8 Rapid Resolution 3.5µm

Courtesy of
The Nest Group, Southborough, MA
Protein Analysis

Proto 300 C4 shows superior efficiency
and more selectivity for impurities than a
214TP52 column

Courtesy of
The Nest Group, Southborough, MA
Glycopeptide Separation

Polyvalent, Unimolecular Glycopeptide Separations

Mono, Di, and Tri Glycan Adducts

Courtesy of
The Nest Group, Southborough, MA
Tryptic Digest of Carbamidomethylated BSA

Proto 200 C18 vs. 238MS5115

16h Tryptic Digest of Carbamidomethylated BSA
20 pMols on column

Courtesy of
The Nest Group, Southborough, MA

 

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